The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 03, 2023
Filed:
Jan. 17, 2019
Applicant:
Tokyo Institute of Technology, Tokyo, JP;
Inventors:
Assignee:
TOKYO INSTITUTE OF TECHNOLOGY, Tokyo, JP;
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 5/09 (2010.01); A61K 9/50 (2006.01); C12N 15/113 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0693 (2013.01); A61K 9/5068 (2013.01); C12N 15/113 (2013.01); C12N 2310/141 (2013.01); C12N 2320/32 (2013.01); C12N 2501/998 (2013.01);
Abstract
The object of the present invention is to provide a method for incorporating an arbitrary protein, lipid, carbohydrate, or nucleic acid into an exosome. The object can be solved by a method for preparing an exosome, comprising the steps of: (a) adding a biological toxin having a perforating activity to a medium containing cells and incubating the mixture, (b) adding ATP and incubating the mixture, and (c) adding a medium containing calcium ion and incubating the mixture.