The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 26, 2023

Filed:

Dec. 05, 2019
Applicant:

Illumina Cambridge Limited, Cambridge, GB;

Inventors:

Pietro Gatti-Lafranconi, Cambridge, GB;

Philip Balding, Cambridge, GB;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6874 (2018.01); B01L 3/00 (2006.01); C12Q 1/25 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6874 (2013.01); B01L 3/502761 (2013.01); C12Q 1/25 (2013.01); C12Q 2527/146 (2013.01); C12Q 2535/00 (2013.01); C12Q 2537/149 (2013.01);
Abstract

Polynucleotide sequencing methods include incubating unlabeled nucleotides with a cluster of template polynucleotide strands having the same sequence when the identity of the previously added labeled nucleotide is being detected. The detection step provides time for the addition of the unlabeled nucleotides to be incorporated into the copy strands in which the previously added labeled nucleotide did not get incorporated. Thus, at the end of the detection step, all or most of the copy strands will be in phase and ready to incorporate the appropriate labeled nucleotide in the subsequence incorporate step.


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