The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 26, 2023
Filed:
May. 04, 2012
Tarjei Mikkelsen, Cambridge, MA (US);
Andreas Gnirke, Wellesley, MA (US);
Alexandre Melnikov, Bellingham, MA (US);
Eric S. Lander, Cambridge, MA (US);
LI Wang, Allston, MA (US);
Xiaolan Zhang, Boston, MA (US);
Tarjei Mikkelsen, Cambridge, MA (US);
Andreas Gnirke, Wellesley, MA (US);
Alexandre Melnikov, Bellingham, MA (US);
Eric S. Lander, Cambridge, MA (US);
Li Wang, Allston, MA (US);
Xiaolan Zhang, Boston, MA (US);
THE BROAD INSTITUTE, INC., Cambridge, MA (US);
Abstract
The invention provides methods for determining the activity of a plurality of nucleic acid regulatory elements. These methods may facilitate, e.g., the systematic reverse engineering, and optimization of mammalian cis-regulatory elements at high resolution and at a large scale. The method may include integration of multiplexed DNA synthesis and sequencing technologies to generate and quantify the transcriptional regulatory activity of e.g., thousands of arbitrary DNA sequences in parallel in cell-based as says (e.g., mammalian cell based assays).