The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 26, 2023

Filed:

Sep. 22, 2020
Applicant:

Abclonal Science Inc., Woburn, MA (US);

Inventors:

Zhenyu Zhu, Lynnfield, MA (US);

Dapeng Sun, Lexington, MA (US);

Michaela Shottes, Methuen, MA (US);

Assignee:

ABclonal Science, Inc., Woburn, MA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); C12N 9/22 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1013 (2013.01); C12N 9/22 (2013.01); C12N 15/102 (2013.01); C12N 15/1065 (2013.01); C07K 2319/00 (2013.01);
Abstract

Disclosed is a product and process, wherein one adds a N-terminal Histamine-Maltose Binding Protein ('MBP') tag to endonucleases, including restriction endonucleases like Hind III, and binds the tagged fusion protein to a solid support, preferably beads, once the enzyme has digested oligonucleotides in solution, in order to arrest further digestion. Preferred beads for binding the tagged enzyme are magnetic beads, which can easily be removed from solution by binding to a support and then removing it, or can be accumulated by magnetic attraction in a particular region. More preferred are magnetic beads bound to iminodiacetic acid or nitrilotriacetic acid.


Find Patent Forward Citations

Zhu, Zhenyu. (2023). His-mbp tagged dna endonuclease for facilitated enzyme removal (U.S. Patent No. 11767524). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/11767524

Data source: USPTO (United States Patent and Trademark Office) public patent records. Weekly synchronization. How IDiyas builds this profile

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