Information processing apparatus and microscope system

Abstract

It is possible to more appropriately perform fluorescence separation. An information processing apparatus according to an embodiment includes: a fluorescence signal acquisition unit () that acquires a plurality of fluorescence spectra corresponding to each of a plurality of excitation lights having different wavelengths and irradiated to a fluorescence stained specimen (), the fluorescence stained specimen () being created by staining a specimen () with a fluorescence reagent (); a link unit () that generates a linked fluorescence spectrum by linking at least parts of the plurality of fluorescence spectra to each other in a wavelength direction; a separation unit () that separates the linked fluorescence spectrum into spectra for every fluorescent substance using a reference spectrum including a linked autofluorescence reference spectrum in which spectra of autofluorescent substances in the specimen are linked to each other in the wavelength direction and a linked fluorescence reference spectrum in which spectra of fluorescent substances in the fluorescence stained specimen are linked to each other in the wavelength direction; and an extraction unit () that updates the linked autofluorescence reference spectrum using the spectra for every fluorescent substance separated by the separation unit.