The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 19, 2023
Filed:
Apr. 16, 2020
Applicant:
Roche Sequencing Solutions, Inc., Pleasanton, CA (US);
Inventor:
Brian Christopher Godwin, Livermore, CA (US);
Assignee:
Roche Sequencing Solutions, Inc., Pleasanton, CA (US);
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6853 (2018.01); C12Q 1/6855 (2018.01); C12N 15/10 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6853 (2013.01); C12N 15/1093 (2013.01); C12Q 1/6855 (2013.01);
Abstract
The present invention relates to a kit and a method of linear amplification of a least one nucleic acid target in a sample, said method comprising: (a) contacting each target in the sample with a nucleic acid polymerase and a primer comprising a component preventing copying of the primer by the nucleic acid polymerase; and at least one nuclease blocking nucleotide; (b) generating a primer extension product; (c) preventing priming by the 3'-end of the primer extension product, and (d) repeating steps b) and c) at least once.