The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 19, 2023
Filed:
Jan. 20, 2020
Bright Dairy & Food Co., Ltd., Shanghai, CN;
Jiangnan University, Wuxi, CN;
Long Liu, Wuxi, CN;
Jian Chen, Wuxi, CN;
Miao Wang, Wuxi, CN;
Guocheng Du, Wuxi, CN;
Xiaomin Dong, Wuxi, CN;
Xueqin Lv, Wuxi, CN;
Jianghua Li, Wuxi, CN;
JIANGNAN UNIVERSITY, Wuxi, CN;
BRIGHT DAIRY & FOOD CO., LTD., Shanghai, CN;
Abstract
The disclosure discloses recombinantfor synthesizing e lacto-N-neotetraose yield. The recombinantis obtained by integrating two β-1,4-galactotransferase genes on a genome of a host bacterium168ΔamyE:P-lacY, P-lgtB, P-comK and exogenously expressing a β-1,3-N-glucosaminotransferase gene. Compared with a strain before transformation, the recombinantof the disclosure improves the yield of the synthesized lacto-N-neotetraose from 720 mg/L to 1300 mg/L, laying a foundation for further metabolic engineering transformation offor producing the lacto-N-neotetraose.