The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 19, 2023
Filed:
Nov. 09, 2020
Freshwater Fisheries Research Center of Chinese Academy of Fishery Sciences, Wuxi, CN;
Jun Qiang, Wuxi, CN;
Zheming Cao, Wuxi, CN;
Pao Xu, Wuxi, CN;
Jie He, Wuxi, CN;
Kai Liu, Wuxi, CN;
Gangchun Xu, Wuxi, CN;
Hongxia Li, Wuxi, CN;
Yifan Tao, Wuxi, CN;
Haojun Zhu, Wuxi, CN;
Abstract
The present disclosure provides a combination of antisense RNA sequences and use thereof in the production of tilapia with degenerated sexual organs, belonging to the technical field of molecular biology and reproductive biology, the combination of antisense RNA sequences includes antisense RNA of steroidogenic factors SF1-1 and SF1-2; the nucleotide sequences of Anti-SF1-1-I, Anti-SF1-1-II, Anti-SF1-2-I and Anti-SF1-2-II are set forth in SEQ ID NO:1-SEQ ID NO:4 respectively. The method of the present disclosure introduces antisense RNA fragments into the eggs through the fertilization hole to realize effective and accurate targeted intervention for regulating the gene expression, and the method has the advantages of simple operation, minimal egg damage, high success rate, stable phenotype after breeding, and excellent application prospects.