The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 12, 2023

Filed:

Nov. 08, 2021
Applicant:

Chinese Pla General Hospital, Beijing, CN;

Inventors:

Qiankun Li, Beijing, CN;

Wenzhi Hu, Beijing, CN;

Cuiping Zhang, Beijing, CN;

Xiaobing Fu, Beijing, CN;

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 7/00 (2006.01); C12N 15/113 (2010.01); C12N 15/86 (2006.01); C12N 5/073 (2010.01); C12N 5/0775 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0605 (2013.01); C12N 5/0668 (2013.01); C12N 7/00 (2013.01); C12N 15/113 (2013.01); C12N 15/86 (2013.01); C12N 2310/141 (2013.01); C12N 2501/11 (2013.01); C12N 2501/115 (2013.01); C12N 2510/00 (2013.01); C12N 2740/15021 (2013.01); C12N 2740/15043 (2013.01);
Abstract

The invention discloses a method for constructing functional exosomes capable of efficiently loading specific miRNA. In order to enable the exosome to carry miRNA with specific regulation function more efficiently so as to play a role in targeted regulation more accurately and efficiently, MS2 phage capsid protein is utilized to edit and construct a capture element of a specific miRNA molecule, and placenta mesenchymal stem cells are reprogrammed to enable the secreted exosome to efficiently load a target miRNA molecule, so that the target miRNA molecule is delivered to tissue cells to play a role in effective regulation, and therefore a new strategy is provided for realizing specific precise treatment in the future.


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