The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 12, 2023

Filed:

Jul. 08, 2016
Applicant:

Max-planck-gesellschaft Zur Förderung Der Wissenschaften E.v., Munich, DE;

Inventors:

Nils A. Kulak, Munich, DE;

Garwin Pichler, Munich, DE;

Matthias Mann, Stockdorf, DE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07K 1/16 (2006.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
C07K 1/165 (2013.01); G01N 33/5002 (2013.01);
Abstract

The present invention relates to a method of purifying peptides and/or polypeptides, said method comprising or consisting of: (a) loading a sample comprising peptides and/or polypeptides under acidic or neutral aqueous conditions on mixed-phase solid phase extraction (SPE) material, wherein said material consists of or comprises reversed phase/ion exchange material; (b) washing said mixed-phase SPE material with (ba) an acidic or neutral composition comprising at least 50% (v/v) organic solvent; and/or (bb) an acidic or neutral aqueous solution; and (c) eluting said peptides and/or polypeptides from said mixed-phase SPE material with an alkaline composition comprising at least 50% (v/v) organic solvent.


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