The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 05, 2023

Filed:

Aug. 16, 2019
Applicant:

Arizona Board of Regents on Behalf of Arizona State University, Scottsdale, AZ (US);

Inventors:

Jeffrey LaBelle, Tempe, AZ (US);

Tina Hakimi, Phoenix, AZ (US);

Brittney Cardinell, Lorton, VA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/543 (2006.01); G01N 27/327 (2006.01); G01N 27/02 (2006.01); A61B 5/145 (2006.01); G01N 27/30 (2006.01); G01N 33/74 (2006.01); A61B 5/053 (2021.01); A61B 5/16 (2006.01);
U.S. Cl.
CPC ...
G01N 33/5438 (2013.01); A61B 5/14507 (2013.01); A61B 5/14546 (2013.01); G01N 27/026 (2013.01); G01N 27/301 (2013.01); G01N 27/327 (2013.01); G01N 27/3273 (2013.01); G01N 33/5436 (2013.01); G01N 33/743 (2013.01); A61B 5/053 (2013.01); A61B 5/165 (2013.01); A61B 2562/0215 (2017.08); G01N 2800/7004 (2013.01);
Abstract

Electrochemical impedance-based label-free and rapid biosensor for select bodily fluid biomolecule levels. Monoclonal antibodies to of biomolecule such as cortisol were covalently attached to a 16-mercaptohexadecanoic acid functionalized gold working electrode using zero-length crosslinkers N-(3-dimethylaminopropyl)-N-ethylcarbodiimide and 10 mM N-hydroxysulfosuccinimide. Cortisol was detected in phosphate buffered saline (simulated tear fluid) using a simple ferrocyanide reagent with a lower limit of detection of 18.73 pM and less than 10% relative standard deviation.


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