The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 05, 2023
Filed:
Dec. 25, 2020
Tianjin University of Science and Technology, Tianjin, CN;
Yefu Chen, Tianjin, CN;
Yanrui Ma, Tianjin, CN;
Yongjing Du, Tianjin, CN;
Sen Jiang, Tianjin, CN;
Jinying Ren, Tianjin, CN;
Guo Zhang, Tianjin, CN;
Xinyue Kang, Tianjin, CN;
Peng Zheng, Tianjin, CN;
Xiaole Wu, Tianjin, CN;
Dongguang Xiao, Tianjin, CN;
Xuewu Guo, Tianjin, CN;
Tianjin University of Science and Technology, Tianjin, CN;
Abstract
Astrain with high yield of ethyl butyrate and a construction method and an application thereof are provided. The strain is obtained by over-expressing in the starting strain acetyl coenzyme A acyl transferase gene Erg10, 3-hydroxybutyryl coenzyme A dehydrogenase gene Hbd, 3-hydroxybutyryl coenzyme A dehydratase gene Crt, trans-2-enoyl coenzyme A reductase gene Ter, and alcohol acyl transferase gene AAT. Compared to the starting bacteria not producing ethyl butyrate, the yield of ethyl butyrate of the constructed strain reaches 77.33±3.79 mg/L, the yield of the ethyl butyrate of the strain with double copy expression of gene Ter and gene AAT reaches 99.65±7.32 mg/L, increased by 28.9% compared with the EST strain, and 40.93±3.18 mg/L of ethyl crotonate is unexpectedly produced.