The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 29, 2023

Filed:

Jan. 07, 2020
Applicant:

Suzhou Biosynthetica Co., Ltd, Jiangsu, CN;

Inventors:

Junjun Jiang, Jiangsu, CN;

Junying Fan, Jiangsu, CN;

Feng Tian, Jiangsu, CN;

Xintong Wang, Jiangsu, CN;

Kailin Zhang, Jiangsu, CN;

Zhihao Hu, Castro Valley, CA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 19/30 (2006.01); C12N 1/20 (2006.01); C12N 9/12 (2006.01); C12N 15/70 (2006.01);
U.S. Cl.
CPC ...
C12P 19/305 (2013.01); C12N 1/20 (2013.01); C12N 9/1205 (2013.01); C12N 9/1241 (2013.01); C12N 15/70 (2013.01); C12Y 207/01032 (2013.01); C12Y 207/07015 (2013.01);
Abstract

The present invention provides a recombinant microorganism for producing citicoline and a method for producing citicoline by using the recombinant microorganism, wherein genes for degradation and utilization of citicoline, choline, and phosphocholine are knocked out, In addition, a pyrimidine nucleoside synthesis pathway is genetically engineered to remove feedback inhibition to the synthesis pathway. A yield of more than 20 g/L of citicoline can be obtained with recombinant strains in a 5-liter fermenter by means of a biological fermentation method, achieving industrial mass production with low citicoline production costs and less pollution; therefore, the method is a simple, environmentally friendly and has a relatively high promotion and application value.


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