The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 29, 2023

Filed:

Jun. 20, 2017
Applicant:

Mgi Tech Co., Ltd., Shenzhen, CN;

Inventors:

Lin Yang, Shenzhen, CN;

Guodong Huang, Shenzhen, CN;

Ya Gao, Shenzhen, CN;

Yanyan Zhang, Shenzhen, CN;

Haiping Zhang, Shenzhen, CN;

Fang Chen, Shenzhen, CN;

Haojun Jiang, Shenzhen, CN;

Shaohong Zhang, Shenzhen, CN;

Yuqian Wang, Shenzhen, CN;

Yifan Xie, Shenzhen, CN;

Hui Jiang, Shenzhen, CN;

Assignee:

MGI TECH CO., LTD., Shenzhen, CN;

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12P 19/34 (2006.01); C12N 15/10 (2006.01); C12N 15/11 (2006.01);
U.S. Cl.
CPC ...
C12N 15/1093 (2013.01); C12N 15/11 (2013.01);
Abstract

Provided are a PCR primer pair and an application thereof. The PCR primer pair comprises: a first primer and a second primer, wherein the first primer comprises a first specific sequence and a first random sequence; the first specific sequence is located on end 3' of the first primer, and the first random sequence is located on end 5′ of the first primer; the second primer comprises a second specific sequence and a second random sequence, the second specific sequence is located on end 3′ of the second primer, and the second random sequence is located on end 5′ of the second primer; moreover, the first specific sequence and the second specific sequence are an upstream primer and a downstream primer directed to a target sequence, respectively; the first random sequence and the second random sequence are reverse complementary; a predetermined restriction enzyme cutting site is connected between the first specific sequence and the first random sequence; and a predetermined restriction enzyme cutting site is connected between the second specific sequence and the second random sequence.


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