The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 29, 2023

Filed:

Aug. 04, 2017
Applicant:

Indiana University Research and Technology Corporation, Indianapolis, IN (US);

Inventors:

Mervin C. Yoder, Indianapolis, IN (US);

Nutan Prasain, Marlborough, MA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/0735 (2010.01); C12N 5/071 (2010.01); A61K 35/12 (2015.01);
U.S. Cl.
CPC ...
C12N 5/0606 (2013.01); A61K 35/12 (2013.01); C12N 5/069 (2013.01); C12N 2501/115 (2013.01); C12N 2501/155 (2013.01); C12N 2501/16 (2013.01); C12N 2501/165 (2013.01); C12N 2501/65 (2013.01); C12N 2506/02 (2013.01); C12N 2506/03 (2013.01); C12N 2506/45 (2013.01); C12N 2533/54 (2013.01);
Abstract

The present disclosure relates generally to methods and compositions useful in cell and tissue biology and therapeutics. In particular, an in vitro method for differentiating pluripotent stem cells into KDRNCAMAPLNRmesoderm cells and/or SSEA5KDRNCAMAPLNRmesoderm cells is provided. The disclosed mesoderm cells may be used to generate blood vessels in vivo and/or further differentiated in vitro into endothelial colony forming cell-like cells (ECFC-like cells). Purified human cell populations of KDRNCAMAPLNRmesoderm cells and ECFC-like cells are provided. Test agent screening and therapeutic methods for using the cell populations of the present disclosure are provided.


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