The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 29, 2023

Filed:

Jun. 29, 2020
Applicant:

Jacques Chammas, Walpole, MA (US);

Inventor:

Jacques Chammas, Walpole, MA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01D 17/12 (2006.01); B01D 21/26 (2006.01); B01L 3/00 (2006.01); G01N 33/49 (2006.01);
U.S. Cl.
CPC ...
B01D 17/12 (2013.01); B01D 21/262 (2013.01); B01L 3/50215 (2013.01); G01N 33/491 (2013.01); B01L 2200/026 (2013.01); B01L 2300/0861 (2013.01); B01L 2400/0409 (2013.01); B01L 2400/0478 (2013.01); B01L 2400/0605 (2013.01);
Abstract

The present invention provides an automated system and method to isolate nucleated blood cells from whole blood or bone marrow. A disc mounted to a centrifuge system with spinning rotor is used to manipulate cells by channeling fluids while subjected to high gravitational field. The disc embodies at least two axisymmetric processing stations connected by a circular channel. Each station contains multiple chambers connected by fluidic channels to controllably transfer fluids. First stage separation allows for the isolation of the buffy coat layer while the second stage separation utilizes gradient density fluids to isolate the targeted nucleated cells from the buffy coat layer in the spinning disc.


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