The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 22, 2023

Filed:

Oct. 05, 2018
Applicant:

The Board of Trustees of the University of Illinois, Urbana, IL (US);

Inventors:

Rashid Bashir, Champaign, IL (US);

Anurup Ganguli, Champaign, IL (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12Q 1/6848 (2018.01); B01L 3/00 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6851 (2018.01); C12Q 1/689 (2018.01); G01N 27/414 (2006.01); B03C 1/01 (2006.01); B03C 1/28 (2006.01); B03C 1/30 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/6848 (2013.01); B01L 3/5027 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/689 (2013.01); C12Q 1/6851 (2013.01); G01N 27/4145 (2013.01); B01L 2200/0689 (2013.01); B01L 2300/0861 (2013.01); B01L 2300/0893 (2013.01); B01L 2300/161 (2013.01); B03C 1/01 (2013.01); B03C 1/288 (2013.01); B03C 1/30 (2013.01); B03C 2201/18 (2013.01); B03C 2201/26 (2013.01);
Abstract

Described herein are systems and methods which utilize an array of wells to isolate pathogens and nucleic acid detection techniques to accurately and rapidly detect pathogens in fluid samples, even in very low concentrations, including from solid or semi-solid samples that have been fluidized. The provided systems and methods dry the fluid sample to deposit a fraction of the total volume in a number of wells and perform nucleic acid detection on individual wells to detect even individual pathogens and provide a quantitative analysis of the amount of pathogen within the sample. Also provided are methods and systems for precise delivery of dried materials, including biomolecules that are enzymes of use in the process, to microwells.


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