The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 15, 2023

Filed:

Aug. 20, 2019
Applicants:

Public University Corporation Nagoya City University, Nagoya, JP;

Fujifilm Corporation, Tokyo, JP;

Inventors:

Takahiro Iwao, Nagoya, JP;

Tamihide Matsunaga, Nagoya, JP;

Satoshi Kondo, Nagoya, JP;

Shota Mizuno, Nagoya, JP;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 5/071 (2010.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0679 (2013.01); G01N 33/5014 (2013.01); G01N 33/5044 (2013.01); C12N 2501/115 (2013.01); C12N 2501/119 (2013.01); C12N 2501/415 (2013.01); C12N 2501/999 (2013.01); C12N 2506/45 (2013.01);
Abstract

It is an object to provide a culture method which is capable of maintaining and/or culturing iPS cell-derived intestinal stem cells, while maintaining the properties of intestinal stem cells. The induced pluripotent stem cell-derived intestinal stem cell-like cells are cultured in the presence of a GSK-3β inhibitor, a histone deacetylation inhibitor, and a serum replacement, or in the presence of a GSK-3β inhibitor and a serum replacement. Preferably, the culture is carried out under conditions in which one or more compounds selected from the group consisting of an epidermal growth factor, a TGFβ receptor inhibitor and a fibroblast growth factor are further present.


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