The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 08, 2023

Filed:

Jan. 29, 2021
Applicant:

The Government of the United States of America, As Represented BY the Secretary of the Navy, Arlington, VA (US);

Inventors:

Igor Medintz, Washington, DC (US);

Joyce A. Breger, Washington, DC (US);

Kimihiro Susumu, Washington, DC (US);

Sebastian Diaz, Washington, DC (US);

Jesper Brask, Lyngby, DK;

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q 1/44 (2006.01); C12Q 1/34 (2006.01); C09K 11/56 (2006.01); G01N 21/64 (2006.01); C09K 11/88 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/44 (2013.01); C09K 11/565 (2013.01); C09K 11/883 (2013.01); C12Q 1/34 (2013.01); G01N 21/6428 (2013.01); G01N 2021/6439 (2013.01);
Abstract

Lipase activity can be detected with a biosensor that includes a quantum dot adhered to a construct having a lipase-cleavable ester to attach a fluorophore acceptor configured as a Förster resonance energy transfer (FRET) acceptor to the QD when the construct is bound thereto. Cleavage of the ester by a lipase results in a measurable reduction in FRET. In further embodiments, the cleavable ester can be used to detect esterase activity, or the ester could be replaced with a glycosidic linkage to detect glycoside activity.


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