The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 08, 2023

Filed:

Nov. 10, 2016
Applicant:

Regents of the University of Minnesota, Minneapolis, MN (US);

Inventors:

Maciej Maselko, Falcon Heights, MN (US);

Mike Smanski, Falcon Heights, MN (US);

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 15/63 (2006.01); C12N 9/22 (2006.01); C12N 15/82 (2006.01); C12N 15/81 (2006.01); C12N 1/14 (2006.01); C12N 1/18 (2006.01); C12N 15/80 (2006.01); C07K 14/005 (2006.01);
U.S. Cl.
CPC ...
C12N 15/8218 (2013.01); C12N 1/14 (2013.01); C12N 1/18 (2013.01); C12N 9/22 (2013.01); C12N 15/63 (2013.01); C12N 15/80 (2013.01); C12N 15/81 (2013.01); C12N 15/815 (2013.01); C12N 15/8216 (2013.01); C12N 15/8217 (2013.01); C12N 15/8263 (2013.01); C12N 15/8265 (2013.01); C07K 14/005 (2013.01); C07K 2319/00 (2013.01); C12N 2510/00 (2013.01);
Abstract

This disclosure describes, in one aspect, a cell that includes a biocontainment system. Generally, the biocontainment system includes a coding region whose overexpression decreases growth of the cell, a transcription regulatory region that includes a silent mutation and is operably linked upstream of the coding region, and a polynucleotide that encodes a programmable transcription activator engineered to bind to the transcription regulatory region in the absence of the silent mutation. Thus, in the absence of the silent mutation, the programmable transcription activator induces overexpression of the coding region; in the presence of the silent mutation, the programmable transcription activator does not initiate overexpression of the coding region.


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