The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 08, 2023
Filed:
May. 24, 2021
Applicant:
The Trustees of the University of Pennsylvania, Philadelphia, PA (US);
Inventors:
Martin Lock, Southampton, PA (US);
Mauricio Alvira, Philadelphia, PA (US);
Assignee:
Ths Trustees of the University of Pennsylvania, Philadelphia, PA (US);
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 7/02 (2006.01); C12N 7/00 (2006.01); B01D 15/38 (2006.01); B01J 20/281 (2006.01); C12N 15/864 (2006.01); G01N 21/33 (2006.01); B01J 41/05 (2017.01); B01D 15/16 (2006.01); B01D 15/36 (2006.01); B01J 41/20 (2006.01);
U.S. Cl.
CPC ...
C12N 7/02 (2013.01); B01D 15/3804 (2013.01); B01J 20/281 (2013.01); C12N 7/00 (2013.01); C12N 15/8645 (2013.01); G01N 21/33 (2013.01); B01D 15/166 (2013.01); B01D 15/363 (2013.01); B01J 41/05 (2017.01); B01J 41/20 (2013.01); C12N 2750/14143 (2013.01); C12N 2750/14151 (2013.01);
Abstract
A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.