The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 08, 2023
Filed:
May. 11, 2022
University of Puerto Rico, San Juan, PR (US);
Jose Lasalde-Dominicci, San Juan, PR (US);
Orestes Quesada-Gonzalez, Canovanas, PR (US);
Josue Rodriguez-Cordero, Carolina, PR (US);
Carlos Baez-Pagan, Carolina, PR (US);
Martin Montoya-Zavala, San Juan, PR (US);
UNIVERSITY OF PUERTO RICO, San Juan, PR (US);
Abstract
The invention is a high-throughput voltage screening crystallographic device and methodology that uses multiple micro wells and electric circuits capable of assaying different crystallization condition for the same or different proteins of interest at the same of different voltages under a humidity and temperature controlled environment. The protein is solubilized in a lipid matrix similar to the lipid composition of the protein in the native environment to ensure stability of the protein during crystallization. The invention provides a system and method where the protein is transferred to a lipid matrix that holds a resting membrane potential, which reduces the degree of conformational freedom of the protein. The invention overcomes the majority of the difficulties associated with vapor diffusion techniques and essentially reconstitutes the protein in its native lipid environment under 'cuasi' physiological conditions.