The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 08, 2023
Filed:
Sep. 20, 2022
Anhui Agricultural University, Hefei, CN;
Anhui Wanjiang Vegetable Industry Technology Research Institute Co., Ltd., Ma'anshan City, CN;
Lingyun Yuan, Hefei, CN;
Liting Zhang, Hefei, CN;
Chenggang Wang, Hefei, CN;
Xingxue Huang, Hefei, CN;
Shidong Zhu, Hefei, CN;
Jinlong Zhang, Hefei, CN;
Jinfeng Hou, Hefei, CN;
Xiaoyan Tang, Hefei, CN;
Zegen Zhang, Hefei, CN;
Anhui Agricultural University, Hefei, CN;
Anhui Wanjiang Vegetable Industry Technology Research Institute Co., Ltd., Ma'anshan, CN;
Abstract
The present invention provides a method for improving a one-time seedling rate of microspore embryoids of, including the steps of: spraying 6-BA onto a flower bud of a plant; sterilizing the flower bud with alcohol and HgCl; releasing microspores, filtering and centrifuging to obtain purified microspores; diluting the microspores with a NLN medium, subpackaging into culture dishes, and adding phytic acid; finally, subjecting to heat shock treatment and transferring to culture in the dark until embryoids appear, and then conducting shaking culture; transferring the cultured embryos in a cotyledon stage onto a MS medium for differentiation culture, wherein the culture conditions are: 4° C., 14 h of illumination by blue-red compound light/day, and 14 days of culture; and then continuing to culture under the condition of 25° C. and 14 h of illumination by blue-red compound light/day until seedlings.