The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 01, 2023

Filed:

Dec. 01, 2020
Applicant:

Illumina Cambridge Limited, Cambridge, GB;

Inventors:

Natalie Morrell, Cambridge, GB;

Andrew Slatter, Cambridge, GB;

Vicki Thomson, Cambridge, GB;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G06F 16/58 (2019.01); G06V 10/26 (2022.01); B01L 3/00 (2006.01); B01L 7/00 (2006.01); C12Q 1/6844 (2018.01); G06T 5/50 (2006.01); C12Q 1/6855 (2018.01); C12Q 1/6853 (2018.01);
U.S. Cl.
CPC ...
G06F 16/58 (2019.01); B01L 3/502761 (2013.01); B01L 7/52 (2013.01); C12Q 1/6844 (2013.01); G06T 5/50 (2013.01); G06V 10/26 (2022.01); B01L 2300/0816 (2013.01); B01L 2300/0819 (2013.01); B01L 2300/0877 (2013.01); B01L 2300/0893 (2013.01); C12Q 1/6853 (2013.01); C12Q 1/6855 (2013.01); C12Q 2304/10 (2013.01); C12Q 2304/12 (2013.01); C12Q 2304/13 (2013.01);
Abstract

In an example method, a series of time-based clustering images is generated for a plurality of library fragments from a genome sample. Each time-based clustering image in the series is sequentially generated. To generate each time-based clustering image in the series: i) a respective sample is introduced to a flow cell, the respective sample including contiguity preserved library fragments of the plurality of library fragments, wherein the contiguity preserved library fragments are attached to a solid support or are attached to each other; ii) the contiguity preserved library fragments are released from the solid support or from each other; iii) the contiguity preserved library fragments are amplified to generate a plurality of respective template strands; iv) the respective template strands are stained; and v) the respective template strands are imaged.


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