The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 01, 2023

Filed:

Aug. 03, 2021
Applicant:

President and Fellows of Harvard College, Cambridge, MA (US);

Inventors:

Evan R. Daugharthy, Cambridge, MA (US);

George M. Church, Brookline, MA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6876 (2018.01); C12Q 1/682 (2018.01); C12N 15/115 (2010.01); C12Q 1/6806 (2018.01); C12Q 1/6816 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6876 (2013.01); C12N 15/115 (2013.01); C12Q 1/682 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6816 (2013.01); C12N 2310/16 (2013.01); C12N 2320/10 (2013.01);
Abstract

The disclosure provides a method for detecting a target analyte in a biological sample including contacting the sample with one or more probe sets each comprising a primary probe and a linker, contacting the sample with an initiator sequence, contacting the sample with a plurality of fluorescent DNA hairpins, wherein the probe binds the target molecule, the linker connects the probe to the initiator sequence, and wherein the initiator sequence nucleates with the cognate hairpin and triggers self-assembly of tethered fluorescent amplification polymers, and detecting the target molecule by measuring fluorescent signal of the sample.


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