The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 01, 2023

Filed:

Dec. 07, 2020
Applicant:

Synaffix B.v., Oss, NL;

Inventors:
Assignee:

SYNAFFIX B.V., Oss, NL;

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 9/24 (2006.01); A61K 47/54 (2017.01); C07K 1/13 (2006.01); C07K 16/18 (2006.01); C12N 15/52 (2006.01); C12N 15/62 (2006.01); C12N 15/66 (2006.01); C12P 21/00 (2006.01);
U.S. Cl.
CPC ...
C12N 9/2497 (2013.01); A61K 47/549 (2017.08); C07K 1/13 (2013.01); C07K 16/18 (2013.01); C12N 9/24 (2013.01); C12N 15/52 (2013.01); C12N 15/62 (2013.01); C12N 15/66 (2013.01); C12P 21/005 (2013.01); C12Y 302/01096 (2013.01); C07K 2319/00 (2013.01);
Abstract

The invention concerns fusion proteins, wherein two endoglycosidases are fused, possibly via a linker. The fusion enzymes according to the invention have structure (1): EndoX-(L)-EndoY (1), wherein EndoX is an endoglycosidase, EndoY is an endoglycosidase distinct from EndoX, L is a linker and p is 0 or 1. Such fusion enzymes capable of trimming glycoproteins comprising at least two distinct glycoforms in a single step. The invention further concerns the use of the fusion enzyme according to the invention for trimming glycoproteins. In another aspect, the invention relates to the process of production of the fusion enzyme. In a further aspect, the inventions concerns a process for trimming glycoproteins, comprising trimming the glycoprotein with a fusion enzyme according to the invention, to obtain a trimmed glycoprotein.


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