The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 01, 2023
Filed:
Jun. 16, 2020
Applicants:
Biolamina Ab, Sundyberg, SE;
The University of Edinburgh, Edinburgh, GB;
Inventors:
Louise Kristina Hagbard, Sollentuna, SE;
Carl Gunnar Jesper Ericsson, Hagersten, SE;
Katherine Rachel Cameron, Edinburgh, GB;
David Colin Hay, Midlothian, GB;
Stuart John Forbes, East Lothian, GB;
Hassan Rashidi, Edinburgh, GB;
Assignee:
BIOLAMINA AB, Sundyberg, SE;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 5/095 (2010.01); C12N 5/074 (2010.01); C12N 5/071 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0695 (2013.01); C12N 5/067 (2013.01); C12N 5/0671 (2013.01); C12N 5/0696 (2013.01); C12N 2500/25 (2013.01); C12N 2500/38 (2013.01); C12N 2500/62 (2013.01); C12N 2501/115 (2013.01); C12N 2501/12 (2013.01); C12N 2501/16 (2013.01); C12N 2501/237 (2013.01); C12N 2501/39 (2013.01); C12N 2501/415 (2013.01); C12N 2501/727 (2013.01); C12N 2501/999 (2013.01); C12N 2506/02 (2013.01); C12N 2513/00 (2013.01); C12N 2533/40 (2013.01); C12N 2533/52 (2013.01); C12N 2533/76 (2013.01); C12N 2533/90 (2013.01);
Abstract
Methods for producing hepatocytes from pluripotent human stem cells are disclosed herein. The stem cells are plated on a cell culture substrate comprising two laminins. The stem cells are then exposed to different cell culture mediums to induce differentiation. The resulting hepatocytes have higher metabolic capacity compared to hepatocytes cultured on different substrates.