The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 25, 2023
Filed:
Jun. 17, 2019
Calico Life Sciences Llc, South San Francisco, CA (US);
Andrew Gregory York, San Francisco, CA (US);
Maria Del Mar Ingaramo, San Francisco, CA (US);
CALICO LIFE SCIENCES LLC, South San Francisco, CA (US);
Abstract
Methods and apparatus for inferring protein binding based on rotational diffusion of a collection of fluorophores. One example of a method includes applying a first light pulse to excite a plurality of fluorophores in the collection of fluorophores to produce a plurality of excited fluorophores, the first light pulse having a first polarization and the plurality of excited fluorophores having a component of their orientation aligned with the first polarization, applying a second light pulse to stimulate emission by the plurality of excited fluorophores, the second light pulse having a second polarization orthogonal to the first polarization, after a time delay following application of the second light pulse, applying a third light pulse of the second polarization to further stimulate emission by the plurality of excited fluorophores, detecting polarized emissions from the plurality of excited fluorophores, and inferring the rate of rotational diffusion of the collection of fluorophores based on the detected polarized emissions.