The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 04, 2023
Filed:
Jan. 28, 2020
Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing, CN;
Han Hu, Beijing, CN;
Qiaohong Wei, Beijing, CN;
Si Chen, Wuhan, CN;
Mao Feng, Beijing, CN;
Lifeng Meng, Beijing, CN;
Bin Han, Beijing, CN;
Yu Fang, Beijing, CN;
Chuan Ma, Beijing, CN;
Jianke Li, Beijing, CN;
Abstract
Match-paired monoclonal antibodies against major royal jelly protein 4 (MRJP4) are secreted by hybridoma cell lines having microbial deposit numbers of CGMCC No. 17294 and CGMCC No. 17295, which are used in an ELISA kit and a colloidal gold immunoassay strip for detecting the MRJP4. The positive and MRJP4-specific cell lines are obtained by cell fusion using an antigen of MRJP4 recombinant protein and a cross-reaction with other major royal jelly proteins. The MRJP4 recombinant protein is used as an antigen to obtain several positive cell lines by cell fusion and two MRJP4-specific fusion cell lines are obtained by a cross-reaction with other major royal jelly proteins. Primary screening of a matched antibody pair is performed according to antibody pairing for recognizing different epitopes.