The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 04, 2023
Filed:
Jul. 30, 2021
Shandong University, Shandong, CN;
Shandong Institute for Food and Drug Control, Shandong, CN;
Avanc Pharmaceutical Co., Ltd, Liaoning, CN;
Ruiqing Xian, Shandong, CN;
Feng Shi, Shandong, CN;
Liping Gong, Shandong, CN;
Lianli Chi, Shandong, CN;
Qunye Zhang, Shandong, CN;
Weijian Wang, Shandong, CN;
Hongming Du, Liaoning, CN;
Congcong Wang, Shandong, CN;
Baojian Hang, Shandong, CN;
Fengshan Wang, Shandong, CN;
Shandong University, Shandong, CN;
Shandong Institute for Food and Drug Control, Shandong, CN;
Avanc Pharmaceutical Co., Ltd, Liaoning, CN;
Abstract
The present invention provides a snake venom thrombin marker peptide ofand an application of the snake venom thrombin-like enzyme in identifying species of Hemocoagulase for Injection. The application includes the following steps of: dissolving a to-be-detected sample and a reference substance of the marker peptide respectively to prepare a test solution and a reference solution, and conducting alkylation reduction on the test solution and the reference solution with dithiothreitol and iodoacetamide; after diluting products with an ammonium bicarbonate solution, adding enzyme for hydrolysis; and after enzymolysis is finished, conducting centrifugation at a high speed, and injecting a supernatan into a liquid chromatography-mass spectrometer for analysis. This method is simple, convenient and rapid, is strong in specificity, fills the gap in identifying the source of species of the snake venom thrombin-like enzyme ofand improves the quality control level.