The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 27, 2023
Filed:
Feb. 06, 2020
Applicant:
Biomadison, Inc., Del Mar, CA (US);
Inventor:
Ward C Tucker, Monona, WI (US);
Assignee:
BioMadison, Inc., Del Mar, CA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/85 (2006.01); C12Q 1/37 (2006.01); C07K 14/435 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/37 (2013.01); C07K 14/43595 (2013.01); C07K 2319/03 (2013.01); C07K 2319/50 (2013.01); C07K 2319/60 (2013.01); G01N 2333/952 (2013.01);
Abstract
Compositions and methods are disclosed that provide a rapid, sensitive, and accurate cell-based assay for enzyme activity, particularly for enzyme activities associated withtoxins. A cell is provided that expresses a construct that includes an anchor region, a cleavage site, and a reporting region having two or more identical reporter peptides. Enzymatic activity at the cleavage site releases the reporter region into the cytosol, where multiple degradation events occur. The observed change in the signal is proportional to the enzymatic activity.