The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 27, 2023

Filed:

Apr. 14, 2021
Applicants:

Chant Oil Co., Ltd., New Taipei, TW;

National Taiwan Normal University, Taipei, TW;

Inventors:

Guan-Chiun Lee, Taipei, TW;

Ting-Chun Kuo, New Taipei, TW;

Shun-Niang Chen, Yilan County, TW;

Chia-Chi Lin, Hsinchu, TW;

Assignees:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12P 7/6418 (2022.01); C12N 9/20 (2006.01); C12P 7/6409 (2022.01); C12P 7/6463 (2022.01); C12R 1/72 (2006.01);
U.S. Cl.
CPC ...
C12P 7/6463 (2013.01); C12R 2001/72 (2021.05);
Abstract

A method for producing cis-unsaturated fatty acid includes the operations below. (i) An oil-water mixture is provided, wherein the oil-water mixture includes 1 to 10 parts by weight of oil and 1 part by weight of water. (ii) 0.002 to 0.5 parts by weight of a recombinantlipase 1 (rCRL1) is added into the oil-water mixture. (iii) The oil-water mixture is emulsified. (iv) The emulsified oil-water mixture is hydrolyzed and fatty acid is generated. (v) Oil-water is separated at a temperature of 55° C. to 65° C. and an oil phase layer is extracted. (vi) The cooling and filtering step is performed to obtain cis-unsaturated fatty acid.


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