The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 20, 2023

Filed:

Jul. 23, 2020
Applicant:

University of Florida Research Foundation, Inc., Gainesville, FL (US);

Inventors:

Wallace Gregory Sawyer, Gainesville, FL (US);

Samantha Lauren Marshall, New York, NY (US);

Eric O. McGhee, Gainesville, FL (US);

Alexander McGhee, Philadelphia, PA (US);

Kylie E. Van Meter, Gainesville, FL (US);

Angela Athena Pitenis, Gainesville, FL (US);

Juan Manuel Urueña, Philadelphia, PA (US);

Derek L. Hood, Gainesville, FL (US);

Michael Dougherty, Gainesville, FL (US);

Christian Jobin, Gainesville, FL (US);

Jack E. Famiglietti, Gainesville, FL (US);

Ryan A. Smolchek, Gainesville, FL (US);

Padraic P. Levings, Gainesville, FL (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12M 1/34 (2006.01); C12M 3/00 (2006.01); C12M 3/06 (2006.01); C12M 1/00 (2006.01); C12M 1/36 (2006.01); C12N 5/00 (2006.01); C12M 1/12 (2006.01);
U.S. Cl.
CPC ...
C12M 41/46 (2013.01); C12M 21/08 (2013.01); C12M 23/16 (2013.01); C12M 23/22 (2013.01); C12M 23/44 (2013.01); C12M 25/14 (2013.01); C12M 29/10 (2013.01); C12M 41/48 (2013.01); C12N 5/0062 (2013.01); C12M 41/36 (2013.01);
Abstract

Disclosed herein is a bioreactor system that allows active perfusive flow through a porous support medium enabling 3D growth of biological samples. In some embodiments, the system comprises a sample well filled with a three-dimensional (3D) cell growth medium. The system can further comprise a liquid medium reservoir fluidly connected to the sample well by a first filter material. The system can further comprises a medium collection chamber fluidly connected to the sample well by a second filter material. In some embodiments, application of negative gage pressure to the medium collection chamber or positive pressure to the liquid medium reservoir draws fluid from the liquid medium reservoir, through the first filter material, into the sample well where it permeates the three-dimensional cell growth medium, through the second filter material, and finally into the medium collection chamber.


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