The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 13, 2023

Filed:

Apr. 08, 2020
Applicant:

Panasonic Intellectual Property Management Co., Ltd., Osaka, JP;

Inventors:

Yumiko Kato, Osaka, JP;

Yoshihide Sawada, Tokyo, JP;

Taichi Sato, Kyoto, JP;

Kiyotaka Tsuji, Osaka, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/483 (2006.01); G01N 21/17 (2006.01); G06T 7/62 (2017.01); G06T 7/11 (2017.01); G06T 7/557 (2017.01); G06T 7/00 (2017.01); G06T 15/00 (2011.01);
U.S. Cl.
CPC ...
G01N 21/17 (2013.01); G06T 7/0012 (2013.01); G06T 7/11 (2017.01); G06T 7/557 (2017.01); G06T 7/62 (2017.01); G06T 15/00 (2013.01); G01N 33/4836 (2013.01); G06T 2207/10056 (2013.01); G06T 2207/30024 (2013.01);
Abstract

The present disclosure provides a technique which makes it possible to evaluate a state of a cell aggregation of one or more spheroids. In the culture state determination device according to the present disclosure, a plurality of light sources sequentially illuminate a plurality of cell aggregations put on an image sensor. The image sensor acquires captured images of the plurality of the cell aggregations each time when the plurality of the light sources illuminate the plurality of the cell aggregations. Control circuitry extracts a region including an image of the cell aggregation in the captured image; generates three-dimensional image information of the region using a plurality of the captured images; extracts an outer shape of the cell aggregation and a cavity part inside the cell aggregation using the three-dimensional image information; calculates a first volume that is a volume based on the outer shape of each of the cell aggregation and a second volume that is a volume of the cavity part based on the cavity part of each of the cell aggregation in the three-dimensional image information; and determines a culture state of the cell aggregations using the first volume and the second volume.


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