The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 13, 2023
Filed:
Mar. 13, 2018
Applicant:
President and Fellows of Harvard College, Cambridge, MA (US);
Inventors:
Suhani D. Vora, Cambridge, MA (US);
Alejandro Chavez, New York, NY (US);
Jing Cheng, Mountain View, CA (US);
Benjamin W. Pruitt, Cambridge, MA (US);
Assignee:
President and Fellows of Harvard College, Cambridge, MA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07H 21/04 (2006.01); C12N 15/11 (2006.01); C07K 14/31 (2006.01); C07K 14/315 (2006.01); C12N 9/22 (2006.01); C12N 15/86 (2006.01);
U.S. Cl.
CPC ...
C12N 15/111 (2013.01); C07K 14/31 (2013.01); C07K 14/315 (2013.01); C12N 9/22 (2013.01); C12N 15/86 (2013.01); C07K 2319/33 (2013.01); C12N 2310/20 (2017.05); C12N 2750/14143 (2013.01);
Abstract
The present disclosure provides methods and compositions of modulating expression of a target nucleic acid sequence in a cell. The method comprises introducing into the cell a nucleic acid sequence encoding a Cas9 fusion protein and a guide RNA, wherein the Cas9 fusion protein and the guide RNA are expressed and co-localize at a target site and modulate the expression of the target nucleic acid sequence.