The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 13, 2023
Filed:
Feb. 19, 2016
Applicant:
Wisconsin Alumni Research Foundation, Madison, WI (US);
Inventors:
James A. Thomson, Madison, WI (US);
Jue Zhang, Madison, WI (US);
Assignee:
Wisconsin Alumni Research Foundation, Madison, WI (US);
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/071 (2010.01); G01N 33/50 (2006.01); A61K 35/44 (2015.01); C12N 5/00 (2006.01);
U.S. Cl.
CPC ...
C12N 5/069 (2013.01); A61K 35/44 (2013.01); C12N 5/0031 (2013.01); C12N 5/0056 (2013.01); G01N 33/5064 (2013.01); C12N 2500/35 (2013.01); C12N 2500/90 (2013.01); C12N 2500/98 (2013.01); C12N 2501/10 (2013.01); C12N 2501/113 (2013.01); C12N 2501/15 (2013.01); C12N 2501/155 (2013.01); C12N 2501/16 (2013.01); C12N 2501/165 (2013.01); C12N 2501/415 (2013.01); C12N 2501/42 (2013.01); C12N 2506/02 (2013.01); C12N 2506/45 (2013.01);
Abstract
Methods for generating human arterial endothelial cells under defined conditions in the absence of insulin are described. In particular, provided herein are efficient, defined, and scalable methods for generating human arterial endothelial cells from human pluripotent stem cells. Also provided herein are uses of human arterial endothelial cells obtained according to these methods. For example, methods of treating peripheral arterial disease and methods of screening agents for that effect adhesion of leukocytes to arterial endothelial cells are also provided.