The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 06, 2023
Filed:
May. 13, 2016
Applicant:
Glaxo Group Limited, Middlesex, GB;
Inventors:
Gary Brian Finka, Stevenage, GB;
Michael Hoare, London, GB;
Mark Uden, Stevenage, GB;
Ioannis Voulgaris, London, GB;
Assignee:
Glaxo Group Limited, Middlesex, GB;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 1/38 (2006.01); C12P 21/02 (2006.01); C07K 16/00 (2006.01); C07K 16/28 (2006.01); C07K 1/14 (2006.01);
U.S. Cl.
CPC ...
C12N 1/38 (2013.01); C07K 16/00 (2013.01); C07K 16/2878 (2013.01); C12P 21/02 (2013.01); C07K 1/145 (2013.01); C07K 2317/14 (2013.01); C07K 2317/569 (2013.01);
Abstract
The present invention relates to a method of producing a recombinant protein in a host cell comprising adding Polyethyleneimine (PEI) during cell culture. Addition of PEI to the cell culture as a fermentation enhancer can result in reducing the viscosity of the cell culture, and/or increasing the extracellular concentration of the recombinant protein, and/or reducing the duration of cell culture to the point of harvest or protein recovery.