The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 06, 2023

Filed:

Jan. 22, 2019
Applicant:

Berkeley Lights, Inc., Emeryville, CA (US);

Inventors:

Kevin D. Loutherback, Berkeley, CA (US);

Yelena Bronevetsky, San Francisco, CA (US);

Peter J. Beemiller, Emeryville, CA (US);

Xiaohua Wang, Albany, CA (US);

Kevin T. Chapman, Santa Monica, CA (US);

Assignee:

Berkeley Lights, Inc., Emeryville, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
B01L 3/00 (2006.01); A61K 35/17 (2015.01); G01N 1/34 (2006.01); B03C 5/02 (2006.01); A61K 35/28 (2015.01); B03C 5/00 (2006.01); G01N 1/40 (2006.01);
U.S. Cl.
CPC ...
B01L 3/502746 (2013.01); A61K 35/17 (2013.01); A61K 35/28 (2013.01); B01L 3/502761 (2013.01); B01L 3/502792 (2013.01); B03C 5/005 (2013.01); B03C 5/026 (2013.01); G01N 1/34 (2013.01); B01L 3/502784 (2013.01); B01L 2200/0652 (2013.01); B01L 2300/0645 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/12 (2013.01); B01L 2300/165 (2013.01); B01L 2400/0424 (2013.01); B01L 2400/086 (2013.01); B03C 2201/26 (2013.01); G01N 2001/4088 (2013.01);
Abstract

Methods of sorting T lymphocytes in a microfluidic device are provided. The methods can include flowing a fluid sample comprising T lymphocytes through a region of a microfluidic device that contains an array of posts. The array of posts can be configured to have a critical size (D) that separates activated T lymphocytes from naïve T lymphocytes. Also provided are microfluidic devices having an array of posts configured to separate activated T lymphocytes from naïve T lymphocytes, compositions enriched for T lymphocytes, particularly activated T lymphocytes that are known to be reactive to an antigen of interest, and methods of treating subjects suffering from a pathogenic disorder or cancer by administering such compositions.


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