The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 06, 2023
Filed:
Dec. 18, 2017
Applicant:
Inje University Industry-academic Cooperation Foundation, Gimhae-si Gyeongsangnam-do, KR;
Inventors:
Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
A61L 27/52 (2006.01); A61L 27/38 (2006.01); C12N 5/00 (2006.01); C12N 5/0797 (2010.01); A61L 27/26 (2006.01); A61K 35/30 (2015.01); A61L 27/36 (2006.01); A61L 27/22 (2006.01); A61L 27/24 (2006.01); A61L 27/54 (2006.01); C12N 5/0735 (2010.01); A61P 29/00 (2006.01);
U.S. Cl.
CPC ...
A61L 27/52 (2013.01); A61K 35/30 (2013.01); A61L 27/225 (2013.01); A61L 27/24 (2013.01); A61L 27/26 (2013.01); A61L 27/3604 (2013.01); A61L 27/3633 (2013.01); A61L 27/3658 (2013.01); A61L 27/3834 (2013.01); A61L 27/54 (2013.01); C12N 5/0068 (2013.01); C12N 5/0606 (2013.01); C12N 5/0623 (2013.01); A61P 29/00 (2018.01); C12N 2501/117 (2013.01); C12N 2501/119 (2013.01); C12N 2501/13 (2013.01); C12N 2501/135 (2013.01); C12N 2501/15 (2013.01); C12N 2501/165 (2013.01); C12N 2501/17 (2013.01); C12N 2501/231 (2013.01); C12N 2501/2306 (2013.01); C12N 2533/52 (2013.01); C12N 2533/54 (2013.01); C12N 2533/56 (2013.01); C12N 2533/90 (2013.01);
Abstract
A method of manufacturing a multilayered cell sheet of neural crest stem cells (NCSCs), includes: (1) isolating and culturing NCSCs from peripheral nerves; (2) embedding the cultured NCSCs in a hydrogel; (3) culturing the hydrogel comprising the NCSCs embedded therein under stressed culture conditions in which a physical support is applied; and (4) culturing the resulting hydrogel of step (3) under non-stressed culture conditions in which a physical support is removed.