The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 30, 2023

Filed:

Jan. 11, 2019
Applicant:

Kao Corporation, Tokyo, JP;

Inventors:

Shenghao Liu, Wakayama, JP;

Yasushi Kageyama, Wakayama, JP;

Mika Terai, Sakai, JP;

Assignee:

Kao Corporation, Tokyo, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/75 (2006.01); C07K 14/32 (2006.01);
U.S. Cl.
CPC ...
C12N 15/75 (2013.01); C07K 14/32 (2013.01);
Abstract

Provided is a method for intracellularly producing a large amount of a Cry protein in abacterium. A method for producing a Cry protein or a culture product comprising the Cry protein, comprising transforming abacterium with an expression plasmid incorporating a gene encoding the Cry protein operably linked to a regulatory region comprising a σA-dependent promoter or a σH-dependent promoter, and culturing the transformed cell, wherein the expression plasmid comprises a polynucleotide encoding a replication protein consisting of the amino acid sequence set forth in SEQ ID NO: 9 or a protein having an identity of 80% or more or more with the amino acid sequence of the replication protein and involved in replication initiation.


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