The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 30, 2023

Filed:

Apr. 13, 2016
Applicant:

The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US);

Inventors:

Billy Tsz Cheong Lau, Palo Alto, CA (US);

Hanlee P. Ji, Stanford, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); C12Q 1/6855 (2018.01); C12Q 1/6806 (2018.01);
U.S. Cl.
CPC ...
C12N 15/1065 (2013.01); C12N 15/1093 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6855 (2013.01); C12Q 2525/191 (2013.01); C12Q 2535/122 (2013.01); C12Q 2563/179 (2013.01); C12Q 2565/514 (2013.01);
Abstract

A population of nucleic acid adaptors is provided. In some embodiments, the population contains at least 50,000 different molecular barcode sequences, where the barcode sequences are double-stranded and at least 90% of the barcode sequences have an edit distance of at least 2. In certain cases, the adaptor may have an end in which the top and bottom strands are not complementary (i.e., may be in the form of a Y-adaptor). In some embodiments and depending on the how the adaptor is going to be employed, the other end of the adaptor may have a ligatable end or may be a transposon end sequence.


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