The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 30, 2023

Filed:

Apr. 07, 2020
Applicant:

Chr. Hansen Hmo Gmbh, Rheinbreitbach, DE;

Inventor:

Stefan Jennewein, Bad Honnef, DE;

Assignee:

Chr. Hansen HMO GmbH, Rheinbreitbach, DE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07H 1/08 (2006.01); A23L 5/00 (2016.01); C07H 3/06 (2006.01); A23L 29/30 (2016.01); A23L 33/00 (2016.01); A23L 33/21 (2016.01); A61K 31/702 (2006.01); C12P 19/14 (2006.01);
U.S. Cl.
CPC ...
C07H 1/08 (2013.01); A23L 5/00 (2016.08); A23L 29/30 (2016.08); A23L 33/21 (2016.08); A23L 33/40 (2016.08); A61K 31/702 (2013.01); C07H 3/06 (2013.01); C12P 19/14 (2013.01); A23V 2002/00 (2013.01);
Abstract

The present application discloses a simple process for the purification of neutral human milk oligosaccharides (HMOs) produced by microbial fermentation. The process uses a combination of cationic ion exchanger treatment, an anionic ion exchanger treatment, and a nanofiltration and/or electrodialysis step, which allows efficient purification of large quantities of neutral HMOs at high purity. Contrary to the purification currently used in fermentative production of neutral HMOs, the presented process allows the provision of HMOs without the need of a chromatographic separation. The so purified HMOs may be obtained in solid form by spray drying, as crystalline material or as sterile filtered concentrate. The provided HMOs are free of proteins and recombinant material originating from the used recombinant microbial strains and thus very well-suited for use in food, medical food and feed (e.g. pet food) applications.


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