The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 23, 2023

Filed:

Aug. 07, 2021
Applicants:

Yi Xiao, Miami, FL (US);

Obtin Alkhamis, Miami, FL (US);

Haixiang Yu, Miami, FL (US);

Inventors:

Yi Xiao, Miami, FL (US);

Obtin Alkhamis, Miami, FL (US);

Haixiang Yu, Miami, FL (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/94 (2006.01); G01N 21/78 (2006.01); G01N 33/58 (2006.01);
U.S. Cl.
CPC ...
G01N 33/9486 (2013.01); G01N 21/78 (2013.01); G01N 33/583 (2013.01); G01N 33/946 (2013.01); G01N 33/948 (2013.01); G01N 33/9413 (2013.01);
Abstract

The subject invention provides materials and methods for single-step detection of target molecules in a sample. The methods and assays of the subject invention employ a dye-displacement strategy, in which aptamers complexed with a cyanine dye for sensitive and rapid detection of targets of interest. In the presence of a target, aptamer-target binding liberates the non-covalently bound aptamer-binding dye, resulting in optical changes that can be observed spectrophotometrically or with the naked eye. The methods and assays of the subject invention enable the colorimetric detection of targets of interest regardless of their structure, sequence, target-binding affinity, and physicochemical properties of their targets.


Find Patent Forward Citations

Loading…