The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 23, 2023

Filed:

May. 16, 2022
Applicant:

Nanjing Haohe Biotechnology Co., Ltd., Nanjing, CN;

Inventors:

Hao Liu, Nanjing, CN;

Qing Xu, Nanjing, CN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 7/46 (2006.01); C12N 15/80 (2006.01); C12N 9/00 (2006.01); C07K 9/00 (2006.01); C07K 14/38 (2006.01); C12N 9/02 (2006.01);
U.S. Cl.
CPC ...
C12P 7/46 (2013.01); C07K 14/38 (2013.01); C12N 9/001 (2013.01); C12N 15/80 (2013.01); C12Y 103/00 (2013.01);
Abstract

The disclosure discloses an Aspergillus niger engineered strain for reducing byproduct succinic acid in a fermentation process of L-malic acid. The Aspergillus niger engineered strain is an Aspergillus niger engineered strain in which fumaric acid reductase frdA and fumaric acid reductase flavoprotein subunit frdB are simultaneously knocked out. The disclosure provides an frdA and frdB gene double-knockout Aspergillus niger strain, and a method for greatly reducing byproduct succinic acid in a fermentation process of L-malic acid. By the disclosure, the byproduct succinic acid accumulated in a production process of malic acid through fermentation of Aspergillus niger is significantly reduced, a cost in a downstream separation and purification process of malic acid is decreased, and good strains are provided for producing malic acid via industrial fermentation.


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