The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 16, 2023

Filed:

Jan. 21, 2020
Applicant:

Quest Diagnostics Investments Incorporated, Wilmington, DE (US);

Inventors:

Brett Holmquist, Mission Viejo, CA (US);

Nigel Clarke, Vista, CA (US);

Anne Caston, San Pedro, CA (US);

Richard E. Reitz, Las Vegas, NV (US);

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N 27/62 (2021.01); G01N 33/82 (2006.01);
U.S. Cl.
CPC ...
G01N 33/82 (2013.01); Y10T 436/10 (2015.01); Y10T 436/20 (2015.01); Y10T 436/203332 (2015.01); Y10T 436/24 (2015.01);
Abstract

Provided are methods of detecting the presence or amount of a dihydroxyvitamin D metabolite in a sample using mass spectrometry. The methods generally comprise ionizing a dihydorxyvitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. In certain preferred embodiments the methods include immunopurifying the dihydroxyvitamin D metabolites prior to mass spectrometry. Also provided are methods to detect the presence or amount of two or more dihydroxyvitamin D metabolites in a single assay.


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