The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 16, 2023

Filed:

Sep. 08, 2016
Applicants:

Sumitomo Pharma Co., Ltd., Osaka, JP;

Healios K.k., Tokyo, JP;

Inventors:

Satoshi Ando, Kobe, JP;

Takao Kuroda, Kobe, JP;

Assignees:

SUMITOMO PHARMA CO., LTD., Osaka, JP;

HEALIOS K.K., Tokyo, JP;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
A61K 35/30 (2015.01); C12N 5/079 (2010.01); C12Q 1/02 (2006.01); A61L 27/00 (2006.01); A61K 35/545 (2015.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0621 (2013.01); A61K 35/30 (2013.01); A61K 35/545 (2013.01); A61L 27/00 (2013.01); C12Q 1/02 (2013.01); G01N 33/5014 (2013.01); G01N 33/5044 (2013.01); C12N 2500/90 (2013.01); C12N 2501/115 (2013.01); C12N 2501/999 (2013.01); C12N 2506/03 (2013.01);
Abstract

The present invention provides a method for more efficiently producing retinal pigment epithelial cells from pluripotent stem cells. The method of the present invention for producing retinal pigment epithelial cells includes the following steps: (1) a first step for culturing a pluripotent stem cell in a medium comprising an FGF receptor inhibitor and/or an MEK inhibitor for a period of not more than 30 days, and (2) a second step for culturing the cell obtained in the first step in the presence of a Nodal signal transduction pathway inhibitor and/or a Wnt signal transduction pathway inhibitor to form a retinal pigment epithelial cell.


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