The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 16, 2023

Filed:

May. 26, 2021
Applicants:

Centre National DE LA Recherche Scientifique (Cnrs), Paris, FR;

Institut National DE LA Sante ET DE LA Recherche Medicale (Inserm), Paris, FR;

Nantes Universite, Nantes, FR;

Centre Hospitalier Universitaire DE Nantes, Nantes, FR;

Inventors:

Mathieu Mevel, Nantes, FR;

David Deniaud, Nantes, FR;

Eduard Ayuso, Nantes, FR;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61K 47/69 (2017.01); A61K 48/00 (2006.01); A61K 49/18 (2006.01); C12N 7/00 (2006.01);
U.S. Cl.
CPC ...
A61K 47/6901 (2017.08); A61K 48/0091 (2013.01); A61K 49/1896 (2013.01); C12N 7/00 (2013.01); C12N 2750/14121 (2013.01); C12N 2750/14122 (2013.01); C12N 2750/14133 (2013.01); C12N 2750/14134 (2013.01); C12N 2750/14142 (2013.01); C12N 2750/14143 (2013.01);
Abstract

The invention is directed to the field of gene therapy, i.e. gene delivery into target cells, tissue, organ and organism, and more particularly to gene delivery via viral vectors. The inventors showed that it is possible by chemical coupling to modulate the coupling of a ligand in the surface of the capsid of AAV, for example AAV2 and AAV3b. In particular, the present invention relates to a recombinant Adeno-Associated Virus (rAAV) vector particle having at least one primary amino group contained in the capsid proteins, chemically coupled with at least one ligand L, wherein coupling of said ligand L is implemented through a bond comprising a —CSNH— bond and an optionally substituted aromatic moiety. Particularly, the inventors tested the chemical coupling of mannose ligand on AAV2 for subretinally injection to rats. The present invention further relates to a method for chemically coupling an Adeno-Associated Virus (AAV) vector particle with at least one ligand L and to a Recombinant Adeno-Associated Virus (rAAV) vector particle obtained by said method as well as a pharmaceutical composition comprising it and their corresponding medical use.


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