The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 16, 2023

Filed:

Dec. 29, 2017
Applicants:

Applied Stemcell, Inc., Milpitas, CA (US);

The Regents of the University of Michigan, Ann Arbor, MI (US);

Inventors:

Ruby Yanru Tsai, San Jose, CA (US);

Jie Xu, Fairfax, VA (US);

Yuqing Chen, Superior Township, MI (US);

Jifeng Zhang, Ann Arbor, MI (US);

Ling-Jie Kong, Union City, CA (US);

Assignees:

ASC THERAPEUTICS INC., Milpitas, CA (US);

THE REGENTS OF THE UNIVERSITY OF MICHIGAN, Ann Arbor, MI (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
A01K 67/00 (2006.01); A01K 67/027 (2006.01); C07K 14/47 (2006.01); C12N 9/22 (2006.01); C12N 15/11 (2006.01); C12N 15/90 (2006.01);
U.S. Cl.
CPC ...
A01K 67/0278 (2013.01); C07K 14/4732 (2013.01); C12N 9/22 (2013.01); C12N 15/11 (2013.01); C12N 15/907 (2013.01); A01K 2217/072 (2013.01); A01K 2227/107 (2013.01); A01K 2267/01 (2013.01); C12N 2310/20 (2017.05); C12N 2800/80 (2013.01);
Abstract

Provided is a genetically modified non-human mammal that comprises an anchor DNA sequence inserted at an endogenous locus of a secretory milk protein gene, wherein the anchor DNA sequence comprises a site-specific recombinase recognition site. Also provided is a genetically modified non-human mammal that comprises a transgene inserted at an endogenous locus of a secretory milk protein gene, wherein the transgene encodes a secretory protein and is operably linked to the endogenous promoter of said secretory milk protein gene, and wherein the transgene is flanked by a pair of site-specific recombinase resulting sites. The genetically modified non-human mammals provided can be used for producing the secreted recombinant protein encoded by the transgene from the milk produced by the genetically modified non-human mammals.


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