The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 02, 2023
Filed:
May. 13, 2022
Taixing Dongsheng Bio-tech Co., Ltd, Taixing, CN;
Jiangnan University, Wuxi, CN;
Xinrui Zhao, Wuxi, CN;
Bohan Zhang, Wuxi, CN;
Jingwen Zhou, Wuxi, CN;
Guocheng Du, Wuxi, CN;
Jianghua Li, Wuxi, CN;
Jian Chen, Wuxi, CN;
Fei Yu, Wuxi, CN;
Wei Lu, Taixing, CN;
Yuan Qian, Taixing, CN;
TAIXING DONGSHENG BIO-TECH CO., LTD, Taixing, CN;
JIANGNAN UNIVERSITY, Wuxi, CN;
Abstract
The disclosure discloses a genetically engineered strain for producing porcine myoglobin and fermentation and purification thereof, and belongs to the technical field of genetic engineering. The disclosure realizes efficient secretion and expression of porcine myoglobin by integrating the gene of porcine myoglobin in. On this basis, optimization of the medium and culture conditions of recombinantcan significantly increase the titer of porcine myoglobin, so that the titer can reach 285.42 mg/L under fermenter conditions. In addition, by creatively adding different concentrations of ammonium sulfate to fermentation broth step by step, the purity of myoglobin obtained by final concentration is up to 88.0%, and the purification rate is up to 66.1%. The disclosure realizes efficient expression and high purification of porcine myoglobin from various steps such as synthesis, fermentation and purification of porcine myoglobin, and provides broad prospects for industrial production of porcine myoglobin.