The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 25, 2023

Filed:

Jan. 19, 2018
Applicant:

The General Hospital Corporation, Boston, MA (US);

Inventors:

Akilan Palanisami, Cambridge, MA (US);

Tayyaba Hasan, Arlington, MA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
B01L 3/00 (2006.01); B01L 7/00 (2006.01); G01N 21/64 (2006.01); G01N 21/76 (2006.01); C12Q 1/04 (2006.01);
U.S. Cl.
CPC ...
G01N 21/645 (2013.01); B01L 3/50273 (2013.01); B01L 3/502715 (2013.01); B01L 3/502753 (2013.01); B01L 3/502761 (2013.01); B01L 7/00 (2013.01); C12Q 1/04 (2013.01); C12Y 305/02006 (2013.01); G01N 21/76 (2013.01); B01L 2300/023 (2013.01); B01L 2300/042 (2013.01); B01L 2300/06 (2013.01); B01L 2300/0609 (2013.01); B01L 2300/0654 (2013.01); B01L 2300/12 (2013.01); B01L 2300/168 (2013.01); B01L 2300/1805 (2013.01); B01L 2400/0487 (2013.01); G01N 2021/6471 (2013.01); G01N 2333/195 (2013.01); G01N 2333/986 (2013.01);
Abstract

The present disclosure features portable wide field fluorimeter systems, e.g., in the form of low-cost mobile platforms, and methods to perform fluorometric assays to detect a change in fluorescence intensity in liquid samples, e.g., caused by the presence of a target analyte, e.g., a protein, e.g., an enzyme (e.g., β-lactamase) expressed by a target pathogen in a liquid sample in a point-of-care setting. In some implementations, a portable system for detecting a change in fluorescence intensity in a liquid sample includes a microfluidic device, an optical assembly including an emission filter and one or more lenses, and an analyzer device that collects and processes a fluorescent signal for the detection of a target analyte produced by the target pathogen present in the liquid sample.


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